Fig. 2

Schematic diagram of liposome preparation by conventional method. i Solubilize lipids in a chloroform/methanol solvent system. i) Evaporate the solvent to create a thin lipid film using a rotary evaporator under reduced pressure. iii Hydrated films, in conjunction with buffers (such as PBS), undergo gentle oscillations at 37 °C to generate enormous multilamellar vesicles. iv Homogenize liposomes to generate unilamellar vesicles using sonication or extrusion through a polycarbonate membrane. (Created with BioRender.com)