Fig. 7
From: High-fat stimulation induces atrial structural remodeling via the TPM1/P53/SHISA5 Axis
In vivo animal experiment validation. Optical microscopy (20X) observation of rat atrial myocardial tissue, HE staining to observe tissue morphology, Masson staining to observe the degree of tissue fibrosis, IHC to observe the expression proportion of GRP78 and p-IRE1 proteins in the tissue, fluorescence microscopy (20x) observation of AAV virus transfection efficiency of SHISA5 in rat atrial myocardial tissue (A). WB detection of Collagen I, p-IRE1α, GRP78, MMP2, P62, p-JNK, P53, SHISA5, LC3-II expression in atrial tissue (B). Immunofluorescence observation of P53 protein (Red) expression in PDGFRα-marked cardiac fibroblasts (Green) (Yellow) (C)