Fig. 5
From: High-fat stimulation induces atrial structural remodeling via the TPM1/P53/SHISA5 Axis
TPM1 induces ER stress and autophagy in HCF cells. WB detection of P62, P53, SHISA5, LC3-II, p-IRE1α, p-JNK protein expression after rTPM1 and BafA1 stimulation (A). TEM observation of autophagosomes and endoplasmic reticulum structures in HCF cells with or without rTPM1 stimulation (B). Immunofluorescence co-localization analysis of autophagy levels in HCF cells after rTPM1 and BafA1 stimulation, Scale Bar = 10 μm (C). Immunofluorescence observation of P53 and SHISA5 protein expression in HCF cells after rTPM1 stimulation, Scale Bar = 10 μm (D). WB detection of Collagen I, p-IRE1α, GRP78, MMP2, P62, p-JNK, LC3-II proteins after 4-PBA inhibition of ERS (E)