Fig. 4

Exosomes^TSH downregulate ATGL and upregulate TGF-β1 expression in adipocytes. (A-C) qPCR (A), Western blot (B) and Quantification of WB (C) of ATGL, HSL, DGAT2, and GPAT3 expression in 3T3-L1 adipocytes treated with exosomes^TSH or exosomes^con. (D-F) qPCR (D), Western blot (E) and Quantification of WB (F) of upstream regulatory factors, including TGF-β1, STAT3, FOXO1, PPARγ, and TNF-α, in 3T3-L1 adipocytes treated with exosomes^TSH or exosomes^con, and in adipose tissue from exosomes^TSH-treated mice. Statistical significance: ** P < 0.01, *P < 0.05