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Table 1 Sequences of primers used in this study

From: Fucoidan ameliorates lipid accumulation, oxidative stress, and NF-κB-mediated inflammation by regulating the PI3K/AKT/Nrf2 signaling pathway in a free fatty acid-induced NAFLD spheroid model

Primer

Forward (5’-3’)

Reverse (5’-3’)

Nrf2

AGCCCAGCACATCCAGTCAG

AAACGTAGCCGAAGAAACCTCA

TNF-α

GCTGCACTTTGGAGTGATCG

GCTGCACTTTGGAGTGATCG

IL-6

GTAGTGAGGAACAAGCCAGAGC

TACATTTGCCGAAGAGCCCT

β-actin

CATCCTCACCCTGAAGTACCCC

AGCCTGGATGCAACGTACATG