Table 1 The following antibodies were used
Antibody | Company | Catalog no. | Dilution |
|---|---|---|---|
Anti Pyruvate kinase M2 (PKM2) | Cell Signaling Technology, Danvers, MA, USA | 4053 | 1000 × |
Anti BCL2-Associated X (Bax) | Abcam, Cambridge, UK | ab32503 | 1000 × |
Anti B-cell lymphoma-2 (Bcl-2) | Abcam | ab32124 | 1000 × |
Anti Cleaved Caspase-3 | Cell Signaling Technology | 9661 | 1000 × |
Anti fatty acid synthase (FASN) | Cell Signaling Technology | 3180 | 1000 × |
Anti- PI3 Kinase p110 Beta (p-PI3K) | Abcam | ab151549 | 1000× |
Anti phospho-protein kinase B (p-AKT) | Cell Signaling Technology | 9271 S | 1000× |
Anti phospho-mammalian target of rapamycin (anti p-mTOR) | Abcam | ab109268 | 1000× |
Anti β-Actin | Sigma-Aldrich, St. Louis, USA | A3854 | 50,000× |