Fig. 4
From: SEC14L2 regulates the transport of cholesterol in non-small cell lung cancer through SCARB1
SEC14L2 targets SCARB1 to regulate cholesterol uptake. (A) GSEA analysis was used to enrich functional pathways of the SEC14L2 gene. (B) STRING predicted the protein-protein interaction between SEC14L2 and SCARB1. (C) The expressions of cholesterol transport-related proteins LDLR, SCARB1, and ABCA1 were detected by WB. (D) The cholesterol content in the tumor was detected by a cholesterol detection kit. *P < 0.05 vs. sh-NC. (E and F) WB and the kit were used to detect the expressions of LDLR, SCARB1, ABCA1, and cholesterol content, respectively. *P < 0.05 vs. MβCD-chol + si-NC. (G) The content of cholesterol in A549 and Calu-1 cells after cholesterol depletion was detected using the kit. *P < 0.05 vs. Control, #P < 0.05 vs. MβCD, &P < 0.05 vs. MβCD + MβCD-chol. (H and I) The spatial localization of SEC14L2 and SCARB1 was analyzed by IF. *P < 0.05 vs. si-NC. (J) Co-IP was used to confirm the targeting relationship between SEC14L2 and SCARB1