Fig. 2

Representative chromatograms of DMAB derivatives of C-triol, 7-KC, 5,6-epoxychol, and 27-OHC (100 ng/mL each). Extract ion: C-triol m/z 534.3/132.1 and C-triol D7 m/z 541.3/132.1 at retention time of 1.48Â min; 7-KC m/z 514.3/132.1 and 7-KC D7 m/z 521.3/132.1 at retention time of 1.48Â min; 27-OHC m/z 516.3/132.1 at retention time of 1.24Â min and 5,6-epoxychol m/z 516.3/132.1 at retention time of 1.69Â min