Fig. 2

Impact of TMEM68 expression on breast cancer cell proliferation, migration and invasion. A-D, Cells were seeded into 96-well plates, and cell viability was determined at the indicated post-seeding times by measuring absorbance at 450 nm using the CCK-8 kit assay. n = 4. E, Transwell migration assay towards 20% FBS. Representative micrographs (20×) were taken from the membrane filter (bottom surface of filters) stained with Crystal violet. F, Transwell matrigel invasion assay towards 20% FBS. G and H, Quantification of transwell migration (G) and matrigel invasion (H) assay by counting the number of cells present in the lower compartment, n = 3. Data are presented as the means ± SDs. Asterisks indicate P values: ** P < 0.01, *** P < 0.001