Fig. 4

LPAR3 deficiency attenuates HIF-2α accumulation in hypoxic mouse kidneys. A Scatter plot of the top 20 enriched KEGG pathways of all differentially expressed genes between the hypoxic kidneys of the WT and Lpar3^−/− mice. B Heatmap of the enriched genes associated with the HIF-1 signaling pathway downregulated by LPAR3 deficiency. C Renal HIF-2α protein levels in the WT and Lpar3^−/− mice subjected to normoxic or hypoxic conditions were determined via Western blotting. D HIF-2α mRNA expression was determined by RT‒qPCR. n = 5–6 per group at each time point. The p value was determined by 2-way analysis of variance (ANOVA) followed by a post hoc test. **p < 0.01, ***p < 0.001, ****p < 0.0001 relative to the isogenic normoxia control; ####p < 0.0001 between two genotype groups; mean ± SEM