Fig. 2
Hypoxia-induced renal EPO expression is decreased by LPAR3 deficiency in mice. A LPAR3 knockout efficiency in the kidneys of Lpar3−/− mice was determined via RT‒qPCR. B Volcano plot showing the differentially expressed genes between the WT and Lpar3−/− mouse kidneys after hypoxia exposure (8% O2) for 12 h, and the Epo gene is highlighted, n = 3. C–D Renal EPO mRNA levels (C) and plasma EPO levels (D) after hypoxia exposure for the indicated times. E-F Hematocrit (E) and hemoglobin (F) levels were determined in the WT and Lpar3−/− mice after hypoxia exposure (8% O2) for 24 h (n = 5‒6). The p value was determined by 2-way analysis of variance (ANOVA) followed by a post hoc test. *p < 0.05, **p < 0.01, ****p < 0.0001 relative to the isogenic normoxia control; ##p < 0.01, ###p < 0.001, ####p < 0.0001 between two genotype groups; mean ± SEM