Fig. 3

(A) Western blot analysis of SREBP1C, ChREBP-β, PKA, AMPK, p-AMPK, and β-actin protein expression. (B, C, D, E, F, and G) Box and whisker plots of SREBP1C, ChREBP-β, PKA, AMPK, and p-AMPK relative to β-actin and p-AMPK/AMPK. CS: control group with standard diet; CF: control group with HFrD; FT: HFrD + HIIT; FD: HFrD + VDS; FTD: HFrD + HIIT + VDS. The middle line in the box represents the median, the upper and lower areas of the center box indicate the 75th and 25th percentiles, respectively, and the vertical bars indicate the standard deviation. Means with different superscripts differ significantly, P < 0.05. *: significant difference compared with CS. †: significant difference from CF. Y: significant difference from FD and FT. Ƴ: significant difference from FT